Detection of Coxiella burnetii in Poultry Egg samples in Iran Using Nested PCR Assay
نویسندگان
چکیده
منابع مشابه
Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples.
A nested PCR method was developed for the detection of Coxiella burnetii in human serum samples. Two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kDa outer membrane protein of C. burnetii. The primers amplified the predicted fragments of 21 various strains of C. burnetii but did not react with DNA samples from other microorganisms. T...
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background: q fever is a zoonosis disease caused by an obligate intracellular organism coxiella burnetii. raw milk or dairy products that are produced by unpasteurized milk may contain virulent coxiella burnetii. the objective of this study was to determine the prevalence rate of c. burnetii in bulk milk samples from dairy sheep herds in fars, ghom, kerman, khuzestan and yazd provinces, iran. m...
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Detection of Coxiella burnetii, the etiologic agent of Q fever, is important for diagnosis of Q fever. PCR-based methods have been widely used for the detection mostly because isolation of C. bumetii is time-consuming. Recent reports showed that PCR-positive rates of Q fever infection widely differed. We have evaluated the PCR and nested PCR assays currently used in Japan. The nested PCR assay ...
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Coxiella burnetii is the causative agent of the zoonotic disease Q fever, and ruminants being considered as the main source for human infection. Although the main route of infection in human is inhalation of contaminated aerosols, oral transmission by contaminated raw milk or unpasteurized dairy products is also a possible route of infection. Raw milk or dairy products produced from unpasteuriz...
متن کاملDetection of Bovine Viral Diarrhea Virus Using a Nested RT-PCR Assay in Bulk Milk Samples of Dairy Cattle Herds in Suburb of Mashhad-Iran
Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against...
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ژورنال
عنوان ژورنال: Asian Journal of Animal and Veterinary Advances
سال: 2012
ISSN: 1683-9919
DOI: 10.3923/ajava.2012.273.276